Freezing Processes

Processes are called freezing (cryopreservation) and then resuscitation (thaw). In the IVF laboratory, both male and female gonad cells and the embryos obtained after the procedure are frozen.

Freezing and thawing are controlled by legal regulations. The storage period of all frozen materials is 5 years. Extension of this period may be possible with petitions submitted to the District Health Directorates.

Live cells, embryos or tissues at very low temperatures (-196 ° C) to be protected by special protective substances (cryoprotectan) covered with glass to be frozen (vitrification).

Sperm Freezing

The sperm freezing process is carried out in the andrology laboratory. Both semen samples obtained by ejaculation and testicular sperm samples obtained by operative means (TESE, TESA) can be frozen. Sperm freezing-thawing processes are considered to be normal at around 30 percent loss of vitality.

Before the treatments that damage the gonad cells, such as sperm retrieval, chemotherapy and radiotherapy, can lead to loss of reproductive functions (sperm taking, etc.) and very few sperm (cryptozoospermia) may require sperm freezing.

Cells stored frozen can be recorded in the records under the supervision of a civil servant determined by the Provincial Directorate of Health, in case of death or death of the received person by the request or death.

Sperm freezing, especially in azoospermia cases, possible repetitive in vitro trials of male patients provides great convenience and a new operation will eliminate the stress.

Embryo Freezing

Embryos can be frozen at all levels from the 2-cell stage to the blastocyst stage by vitrification. According to the comparative scientific studies on pregnancy after transfer of frozen-thawed embryos; until now, there has been no increased risk of complications related to pregnancy during pregnancy or postpartum infants.


InWhich Situations is Embryo Freezing Necessary?

  • After the embryo transfer, remaining good quality embryos remain
  • Clinical conditions that occur during the treatment process and that may adversely affect the health of the patient by transfer of embryos (OHSS risk)
  • Clinical conditions that occur during the treatment process and may adversely affect the chance of success (insufficient uterine thickening, early progesterone increase, polyps or cysts)
  • PGD applications to extend the results of genetic examination
  • External health problems, emergency situations, social problems requiring postponement of transfer

Oocyte (Eggs) Freezing

In recent years, successful results have been obtained with vitrification method and it has been started to be applied as standard egg freezing method. In the egg freezing process, the solutions and carriers used in the embryo freezing process are used, but the freezing protocol is different. The egg freezing process is applied about 2 hours after the process of egg collection. This is the ideal time for a successful freezing.
Egg freezing process can be applied within the conditions and limitations determined by the Ministry of Health.

In Which Cases You May Need Egg Freezing?

Prior to treatments that damage gonad cells such as chemotherapy and radiotherapy
Operations that lead to loss of reproductive functions (operations such as removal of the ovaries)
A small number of ovarian ovarian cells (Low Overreservation-DOR) and the case of a family history of early menopause in a family history of three experts in the case of a medical board report is documented


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